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LDH, is only released from apoptotic blebs after secondary necrosis occurs. Pyroptosis is distinct from necrosis in that it requires the activity of caspase-1. In this chapter, we describe enzymatic assays for the ,detection, of ,LDH, released by pyroptotic cells using a commercially available ,kit,, as well as a simple and cost-effective method adapted from Decker et al. (J Immunol Methods 115:61-69 ...
This immunoassay ,kit, allows for the ,in vitro, quantitative determination of Human ,LDH,, concentrations in serum, tissue homogenates and other biological fluids. Test principle The microtiter plate provided in this ,kit, has been pre-coated with an antibody specific to ,LDH,.
LDH, activity, therefore, can be used as an indicator of cell membrane integrity and serve as a general means to assess cell viability by measuring plasma membrane permeability. Cayman's ,LDH, Cytotoxicity Assay ,Kit, measures cell death in response to chemical compounds or environmental factors using a coupled two-step reaction.
This ,kit, uses Lactate Dehydrogenase (,LDH,) to oxidize the excess NADPH. The ,kit, is particularly well suited to measurements of NOS activity ,in vitro, . High throughput screening applications using recombinant nitric oxide synthase (NOS) preparations can be quickly and accurately assayed with the ,LDH, method ,kit,.
The aim of this study was to compare four ,in vitro, cytotoxicity assays and determine their ability to detect early cytotoxic events. Two hepatoma cell lines, namely HTC and HepG2 cells, were exposed to cadmium chloride (0-300 microM) for 3, 5 and 8 h. Following exposure to the toxic metal cytotoxici …
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The Roche ,LDH, Cytotoxicity ,Detection Kit, is designed to be a simple method to directly quantify cell death in culture. The assay is based on the measurement of lactate dehydrogenase (,LDH,) released into the growth media when the integrity of the cell membrane is lost.
LDH Detection, Enzyme Mix 50µl 5ml Reductase Substrate 0.25µl 25µl 3. Mix the ,LDH Detection, Reagent by gently inverting five times. Note: Store unused ,LDH Detection, Enzyme Mix below –65°C or at –30°C to –10°C. Store unused Reductase Substrate below –65°C protected from light. Do not store prepared ,LDH Detection, Reagent.!