Shanghai Sunland Industrial Co., Ltd is the top manufacturer of Personal Protect Equipment in China, with 20 years’experience. We are the Chinese government appointed manufacturer for government power,personal protection equipment , medical instruments,construction industry, etc. All the products get the CE, ANSI and related Industry Certificates. All our safety helmets use the top-quality raw material without any recycling material.
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11/3/2014, · ,LDH, Assay ,Kit, (Cytotoxicity) ab65393 uses WST for the fast and sensitive ,detection, of ,LDH, released from damaged cells. The ,LDH, assay, also known as ,LDH, release assay, is a cell death / cytotoxicity assay used to assess the level of plasma membrane damage in a cell population.
The CyQUANT ,LDH, Cytotoxicity Assay is a colorimetric assay that provides a simple and reliable method for determining cellular cytotoxicity. Lactate dehydrogenase (,LDH,) is a cytosolic enzyme present in many different cell types that is released into the cell culture medium upon damage to the plasma membrane.
Detection, Principle Lactate dehydrogenase(,LDH,) is an enzyme that presents in almost cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once cells are impaired by stress, injuries, chemicals, or intercellular signals, ,LDH, is …
The Cytotoxicity ,Detection Kit, PLUS (,LDH,) is a fast, sensitive, and simple method to quantify cytotoxicity/cytolysis based on the measurement of ,LDH, activity released from damaged cells using the 96-well or 384-well plate format. The ,kit, can be used in many different ,in vitro, cell systems when damage to the plasma membrane occurs.
Detection, Principle Lactate dehydrogenase(,LDH,) is an enzyme that presents in almost cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once cells are impaired by stress, injuries, chemicals, or intercellular signals, ,LDH, …
LDH, is a stable cytoplasmic enzyme present in all cells. Once cells are impaired by stress, injuries, chemicals, or intercellular signals, ,LDH, is rapidly released into the cell culture medium through the damaged plasma membrane. The cytotoxicity ,detection, mechanism of this ,kit, is based on the reduction of NAD+ to NADH when ,LDH, catalyzes ...
Application The Cytotoxicity ,Detection Kit, is designed as a precise, fast and simple colori-metric alternative to quantitate cytotoxicity/cytolysis based on the measure-ment of ,LDH, activity released from damaged cells. Thus, the Cytotoxicity ,Detection Kit, can be used in many different ,in vitro, cell systems when damage to the plasma membrane occurs.
LDH, activity, therefore, can be used as an indicator of cell membrane integrity and serve as a general means to assess cell viability by measuring plasma membrane permeability. Cayman's ,LDH, Cytotoxicity Assay ,Kit, measures cell death in response to chemical compounds or environmental factors using a coupled two-step reaction.